Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 8;13(8):693. doi: 10.1038/s41419-022-05138-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A Representative images of Masson trichrome staining of kidney sections. Kidneys were isolated from Gsdmd^+/+ mice and Gsdmd^−/− mice on day 13 after UUO. Scale bar: 100 μm. B Quantification of renal fibrosis scores evaluated by Masson trichrome staining. n = 6. C, E Representative images of immunofluorescence staining of kidney sections, showing the expression of α-smooth muscle actin (α-SMA) C and type-I collagen (Col I) E. DAPI was used for nuclear staining. Scale bar: 100 μm. D, F Quantification of α-SMA D and Col I F expression by immunofluorescence. n = 6. G Western blot analysis for α-SMA and Col I expression of kidney tissue lysates, which were isolated on day 7 and 13 after UUO. GAPDH was used as a loading control. n = 4. H Western blot showing GSDMD cleavage in kidney after UUO. Kidney tissue lysates were isolated on day 0, 7, and 13 after UUO. The knockout efficiency of Gsdmd was also confirmed, and the cleaved GSDMD-N could be observed in WT kidneys. n = 4. **P < 0.01.