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. 2022 Aug 8;13(8):693. doi: 10.1038/s41419-022-05138-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A–C t-distributed stochastic neighbor embedding (tSNE) plot of cell clusters identified on the basis of the expression of highly variable genes in the kidneys from control group (n = 2), obstructive kidney on day 2 (n = 2) and day 10 (n = 2) after UUO. tSNE analysis showed 11 distinct clusters of renal cells in control A and 13 distinct clusters in UUO C mice. The distribution of cell clusters was consistent between obstructive kidney on day 2 and day 10 B. DCTs: distal convoluted tubules; ECs: endothelial cells; Fibro: fibroblast; Macro: macrophage; Myofibro: myofibroblast; Neutro: neutrophil; NK: natural killer cell; Podo: podocyte; PTCs: proximal tubular cells; DC: dentritic cell; TE: transitional epithelia. D, E Unsupervised trajectory of macrophage-to-myofibroblast transition along pseudotime using Monocle. F Heatmap of top 20 genes significantly changed in macrophage-to-myofibroblast transition in pseudotime. Each row represents a gene. The left and right end corresponds to the starting point (macrophage) and the ending point (myofibroblast) respectively. Color scheme represents the z-score distribution from −3.0 to 3.0. G Network visualization of the ligands expressed by macrophage and the other cells expressing the cognate receptors primed to receive the signal. Numbers indicate the quantity of ligand-receptor pairs for each intercellular crosstalk. H, I Violin plots of log-transformed gene expression of GSDMD in each cell population of control kidney H and obstructive kidney on day 2 and 10 after UUO I. J, K Comparison of gene expression of GSDMD in neutrophils J and macrophages K between control kidney and obstructive kidney on day 2 and 10 after UUO. L Network visualization of the ligands expressed by neutrophil and the other cells expressing the cognate receptors primed to receive the signal. Numbers indicate the quantity of ligand-receptor pairs for each intercellular crosstalk. M Heat map showing the difference of NETs-associated genes expression in neutrophils among control kidney and obstructive kidney on day 2 and 10 after UUO. The colors of yellow to blue represented alterations from high expression to low expression. *P < 0.05; ***P < 0.001.