Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 8;13(8):693. doi: 10.1038/s41419-022-05138-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — A Western blot showing caspase11 activation in the kidneys after UUO. Kidney tissue lysates were isolated on day 0, 2, 5, and 10 after UUO. n = 4. B Representative images of Masson trichrome staining of kidney sections from Casp11^wt/wtMrp8^cre and Casp11^fl/flMrp8^cre mice on day 13 after UUO. Scale bar: 100 μm. n = 6. C, D Representative images of immunofluorescence staining of kidney sections from Casp11^wt/wtMrp8^cre and Casp11^fl/flMrp8^cre mice, showing the expression of α-SMA C and Col I D. DAPI (blue) was used for nuclear staining. Scale bar: 100 μm. E Representative images of immunofluorescence staining of kidney sections from Casp11^wt/wtMrp8^cre and Casp11^fl/flMrp8^cre mice on day 10 after UUO, showing the expression of Histone-H3 and MPO. Scale bar: 100μm. F, G Representative images of immunofluorescence staining of Cit-H3 and MPO and quantification of neutrophils undergoing NETs formation. The neutrophils were isolated from Casp11^+/+ and Casp11^-/- mice and treated as described in Fig. 7A. n = 6. Scale bar: 50 μm. H Schematic model of GSDMD-dependent NETs promoting MMT and renal fibrosis in obstructive nephropathy.