Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 8;11(1):45. doi: 10.1038/s41389-022-00421-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A, C GSN knockdown inhibits cell migration and invasion of HeLa and MGC803 cells. A The protein levels of metastasis-related markers were measured by western blotting in HeLa and MGC803 cells transfected with empty vector, GSNshRNA1 or GSNshRNA2. C Transwell assays were performed to test the migratory and invasive potential of the cells above (n = 4, one-way ANOVA) (A). Scale bar, 200 µm. B, D, E GSN knockdown inhibits cell proliferation in HeLa and MGC803 cells. B The levels of proliferation-related proteins were measured by western blotting in the cells above (A). D MTT (n = 3, one-way ANOVA) and (E) EdU incorporation assays were performed to test the ability of cell growth in the cells above (n = 4, one-way ANOVA) (A). F GSN knockdown promotes filaments formation. F-actin were stained with phalloidin in the cells above (A). G Methylation of HBP1 alleviates its inhibition of filaments formation. HeLa and MGC803 cells were stably overexpressing empty vector, HBP1 or R378A and the levels of F-actin were analyzed by immunofluorescence intensity. Scale bar, 5 µm. H–K Methylation of HBP1 alleviates its inhibition of metastasis and growth of HeLa cells through down-regulating GSN expression. H HeLa cells stably expressing HBP1, R378A, HBP1 + shGSN-1, R378A + shGSN-1, HBP1 + shGSN-2, and R378A + shGSN-2 individually, the protein levels of metastasis-related and proliferation-related markers were measured by western blotting. I Transwell assays were performed to test the migratory and invasive potential of the cells above (n = 4, one-way ANOVA) (H). Scale bar, 200 µm. J MTT (n = 3, one-way ANOVA) and (K) EdU incorporation assays were performed to test the ability of cell growth in the cells above (n = 4, one-way ANOVA) (H). L Methylation of HBP1 promotes filaments formation by downregulating GSN. F-actin were analyzed by immunofluorescence intensity with the cells above (H). Scale bar, 5 µm. Date were the mean ± SD. *p < 0.05, **p < 0.01.

Fig. 6 — A, C GSN knockdown inhibits cell migration and invasion of HeLa and MGC803 cells. A The protein levels of metastasis-related markers were measured by western blotting in HeLa and MGC803 cells transfected with empty vector, GSNshRNA1 or GSNshRNA2. C Transwell assays were performed to test the migratory and invasive potential of the cells above (n = 4, one-way ANOVA) (A). Scale bar, 200 µm. B, D, E GSN knockdown inhibits cell proliferation in HeLa and MGC803 cells. B The levels of proliferation-related proteins were measured by western blotting in the cells above (A). D MTT (n = 3, one-way ANOVA) and (E) EdU incorporation assays were performed to test the ability of cell growth in the cells above (n = 4, one-way ANOVA) (A). F GSN knockdown promotes filaments formation. F-actin were stained with phalloidin in the cells above (A). G Methylation of HBP1 alleviates its inhibition of filaments formation. HeLa and MGC803 cells were stably overexpressing empty vector, HBP1 or R378A and the levels of F-actin were analyzed by immunofluorescence intensity. Scale bar, 5 µm. H–K Methylation of HBP1 alleviates its inhibition of metastasis and growth of HeLa cells through down-regulating GSN expression. H HeLa cells stably expressing HBP1, R378A, HBP1 + shGSN-1, R378A + shGSN-1, HBP1 + shGSN-2, and R378A + shGSN-2 individually, the protein levels of metastasis-related and proliferation-related markers were measured by western blotting. I Transwell assays were performed to test the migratory and invasive potential of the cells above (n = 4, one-way ANOVA) (H). Scale bar, 200 µm. J MTT (n = 3, one-way ANOVA) and (K) EdU incorporation assays were performed to test the ability of cell growth in the cells above (n = 4, one-way ANOVA) (H). L Methylation of HBP1 promotes filaments formation by downregulating GSN. F-actin were analyzed by immunofluorescence intensity with the cells above (H). Scale bar, 5 µm. Date were the mean ± SD. *p < 0.05, **p < 0.01.