Table 2.
Expression of bioactive factors in membrane lysates analyzed by protein microarray
| Factor | Biological activity |
Group 1 (median) | Group 2 (median) | Group 3 (median) | Group 4 (median) |
|---|---|---|---|---|---|
| Angiogenin | A | 106 | 102 | 132 | 97 |
| Angiopoietin-2 | A | 9 | 7 | 8 | 6 |
| Complement component C5/C5a | I | 9 | 7 | 9 | 10 |
| IFN-γ | I | 4 | 3 | 3 | 3 |
| C-reactive protein | I | 82 | 93 | 74 | 79 |
| ENA-78 (CXCL5) | I | 10 | 11 | 15 | 7 |
| IL-1ra | I | 10 | 10 | 7 | 8 |
| IL-8 (CXCL8) | I | 24 | 21 | 33a | 20 |
| IL-17A | I | 7 | 6 | 7 | 7 |
| MCP-1 (CCL2, MCAF) | I | 9 | 9 | 9 | 11 |
| MIF | I | 40 | 38 | 42 | 27 |
| RANTES (CCL5) | I | 14 | 13 | 18 | 14 |
| Chitinase 3-like 1 | R | 95 | 87 | 90 | 83 |
| Endoglin (CD105, ENG) | R | 77 | 71 | 75 | 67 |
| FGF basic | R | 3 | 3 | 3 | 5 |
| IGFBP-2 | R | 6 | 8 | 11 | 7 |
| Osteopontin (OPN) | R | 77 | 73 | 100 | 69 |
Factors were roughly allocated to their main biological activity: angiogenic (A), inflammatory (I), or involved in tissue regeneration (R). Data represent chemiluminescence signal intensities given as median values of respective time groups. Differences of factors in the time groups 1–4 were calculated using one-way analysis of variance (ANOVA) with correction for multiple comparisons (Bonferroni) (p ≤ 0.05)
aStatistical significance between group 3 and group 1, 2 and 4