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. 2022 Jul 6;12(9):2187–2206. doi: 10.1007/s13346-022-01203-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Effects of the organic nanoparticles on cell viability measured in the WST-8 assay performed by the three individual partner laboratories BMTZ, RIVM, and SINTEF. In the bar charts, mean cell viability (%) in comparison to the cell culture medium control for each combination of cell line and organic nanoparticles tested is shown. Standard deviation of mean is indicated by whiskers. Cells were incubated for 24 h with the organic nanoparticles and controls. Subsequently, a WST-8 assay was performed as described in the method section. Data was acquired from three independent experiments (n = 3) with up to 8 technical repeats each (N = 8). An ANOVA was performed for the statistical analysis of the WST-8 assay results in comparison to the cell culture medium control, and significance levels were given as p < 0.005 (***), p < 0.01 (**), and p < 0.05 (*)