The endogenous MIR133A expression in colon cancer tissues and MIR133A-overexpressing CRC cells. A. The expression of MIR133A was validated using six colon cancer tissue samples and matching adjacent healthy colon tissue samples. The relative expression of MIR133A was normalized to colon-specific RNU48. The data are presented as the relative levels (ΔΔCT method) of MIR133A in colon cancer tissue. T1, T2, T3, T4, T5, and T6 indicate the tumor sites of patients with colon cancer. B, C. The relative endogenous MIR133A expression level in MIR133A1 and A2 overexpressed SW48 and HCT116 cell lines. Data are presented as mean ± SD. (**P<0.01, ***P<0.001).