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. 2022 Jul 15;12(7):3223–3241.

Figure 5.

Figure 5

MIR133A regulates SOX9-mediated downstream MAPK pathways in CRC cell lines. A. Western blot analysis of SOX9 and its downstream KRAS-BRAF-MAP2K1-MAP2K1/3-pMAP2K1/3 in HCT116 and SW48 cells transfected with MIR133A mimic. B. SOX9 and its downstream KRAS-BRAF-MAP2K1-MAP2K1/3-pMAP2K1/3 in HCT116 and SW48 cells transfected with siSOX9. The protein levels of respective genes were determined by western blotting and densitometry using Image J, where GAPDH was used as a loading control. Representative data from at least three independent experiments are shown. Each bar represents mean fold alternation above or below control (± SD). Differences were considered statistically significant *P<0.05, **P<0.01 compared with control (ns = not significant).