Figure 7.

MIR133A regulates SOX9 and apoptosis pathways in CRC cell lines. A. Western blot analysis of BCL2, BAX, and cleaved CASP9 in HCT116 and SW48 cells transfected with MIR133A mimic. B. BCL2, BAX, and cleaved CASP9 in HCT116 and SW48 cells transfected with siSOX9. The protein levels of respective genes were determined by western blotting and densitometry using Image J, where GAPDH was used as a loading control. Representative data from at least three independent experiments are shown. Each bar represents mean fold alternation above or below control (± SD). Differences were considered statistically significant *P<0.05, **P<0.01 compared with control (ns = not significant).