MIR133A inhibits cell proliferation, colony formation, and migration by targeting SOX9 in CRC cell lines. A. MTT assays of MIR133A mimic- and siSOX9-transfected in HCT116 and SW48 cell lines. B. Colony formation assay of HCT116 cells transfected with MIR133A mimic and siSOX9. C. The scratch wound assay was conducted in MIR133A mimic-transfected and siSOX9 in HCT116 cells. Migration distance was measured at 0, 24, 48, and 72 hours after the cells were scratched. Representative data from at least three independent experiments are shown. Each bar represents mean fold alternation above or below control (± SD). Differences were considered statistically significant *P<0.05, **P<0.01 compared with control (ns = not significant).