Figure 7.

Knockdown of SHMT2 inhibits breast tumor growth in vivo. HCC1806 cells with SHMT2 knockdown (KD-SHMT2) and control cells (KD-NC), while BT549 cells (SHMT2) with SHMT2 over-expression and control cells (Vector) were injected into the left and right back subcutaneously in 100 ul PBS. The average tumor volume reached 100 mm³ and tumor volume was measured. Xenograft tumors were measured with vernier calipers, tumor volumes were calculated and growth curves were plotted (A, E). After 7 measurements, the mice were sacrificed and photographed (B, F). The xenograft was removed and photographed (C) and weighed (D, G). Immunohistochemical staining (H) detected the protein expression levels of P-ERK, P-P38, KI67, CD31, and SHMT2 in tumor tissues, *P < 0.05, Student’s t-test. The data are presented as mean ± SD from two independent experiments. N = 5 or 6/group, magnification 200 ×.