FIG. 2.

Primer extension analysis of the hpt-specified mRNA. The numbering of the nucleotide sequence is as in Fig. 1, with the coding strand displayed. A 50-μg quantity of RNA prepared from M. thermoautotrophicum was used in each experiment, and results obtained with complete extension mixture (+) and with an extension mixture without reverse transcriptase (as a control) (−) are shown. Lanes G, A, T, and C show the sequences generated using primer 15350 together with plasmid pSAUE 7 as template. The putative transcriptional start sites are marked on the gel with arrows and on the sequence to the left of the gel with asterisks. A putative box A promoter element is boxed on the sequence.