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. 2022 Aug 8;12:105. doi: 10.1186/s13568-022-01446-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Comparative statistical analysis of LCA to UDCA conversion (144 h) by G. zeae parent (P) and mutant (M) strains (GC data of three independent experiments) (a). TLC profiles of 120 h (b) and 144 h (c) samples (~ 10 µg of steroids in the spot): S1, standard samples of LCA (0.5 mkg) and UDCA (7 mkg); S2, standard samples of LCA (1.0 mkg) and UDCA (8 mkg) (top to bottom); X–undefined metabolite (the structure of the product was not determined due to its low amount and concomitant formation of several metabolites). LCA initial concentration was 4 g/L