Fig. 7. Ib30 potentiates agonist-induced ERK1/2 activation for the V₂R^T360A mutant.

a HEK-293 cells expressing the indicated receptor construct together with Ib-CTL/Ib30 were stimulated with AVP (100 nM) followed by detection of ERK1/2 phosphorylation using western blot. The expression of Ib-CTL/Ib30 is monitored using anti-HA antibody. b Densitometry-based quantification from sixteen independent experiments, normalized with respect to V₂R^WT + Ib-CTL condition (treated as 100%) (One-way ANOVA, Sidak’s multiple comparisons test; ***p = 0.0006, ****p < 0.0001, ns = non-significant). The data represented as box plots showing median, IQR with whiskers of 1.5× IQR, and circles represent values from sixteen independent experimental replicates. Source data are provided as a Source Data file.