Fig. 6. D-R pAgos sense mismatches in the RNA target.

a Scheme of the guide-target duplex with indicated functional regions in guide DNA. The cleavage sites by PliAgo and PnyAgo are indicated. b Effects of single-nucleotide mismatches on the efficiency of RNA cleavage by D-R pAgos (see Supplementary Table 1 for guide DNA and target RNA sequences). The reactions were performed with 5’-P³²-labeled target RNA at 37 °C for 60 min for PliAgo, RslAgo, HpeAgo and 10 min for PnyAgo; positions of the target (‘T’) and the reactions products (‘P’) are indicated. Representative gels from three independent experiments are shown (see Supplementary Fig. 9 for means and errors). The positions around the cleavage sites of corresponding pAgo proteins are shown with dotted boxes.