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. 2022 Jul 26;13:940094. doi: 10.3389/fimmu.2022.940094

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Copyright © 2022 Raigani, Santiago, Ohman, Heaney, Baptista, Coe, de Vries, Rosales, Shih, Markmann, Gruppuso, Uygun, Sanders and Yeh

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Liver preservation and NMP protocol. Emricasan (5mg/kg liver) or vehicle control (1mL DMSO) was added to the circulating perfusate immediately prior to connection of the liver to the NMP device. Serial tissue biopsies and perfusate samples were taken at indicated time points for subsequent analysis. * indicates a discordance between EM perfusions (bile samples taken at 3, 6, 9, and 12 hours) and control perfusions (bile samples taken at 4, 8, and 12 hours). NMP, normothermic machine perfusion; WIT, warm ischemic time; CIT, cold ischemic time; RNAseq, bulk RNA sequencing.