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. 2022 Jul 26;13:940094. doi: 10.3389/fimmu.2022.940094

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Copyright © 2022 Raigani, Santiago, Ohman, Heaney, Baptista, Coe, de Vries, Rosales, Shih, Markmann, Gruppuso, Uygun, Sanders and Yeh

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Autophagy and the unfolded protein response during liver NMP. Western immunoblots of (A) phosphorylated to total protein ratio of eiF2α, (B) phosphorylated to total protein ratio P70S6K, (C) Beclin, (D) LC3B-II to LC3B-I ratio, and (E) p62. Kruskal-Wallis test: ## P<0.01, # P<0.05; Wilcoxon ranksum test: ** P<0.01, * P<0.05. NMP, Normothermic machine perfusion; AHF, adequate hepatocellular function; IHF, inadequate hepatocellular function; EM, emricasan; eiF2α, eukaryotic initiation factor 2α; P70S6K, P70 S6 kinase; LC3B, microtubule-associated protein 1 light chain 3 beta.