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Primer extension analysis of acs1 with oligonucleotide 5-1 (5′ CACCAGCCAAAATGATCC). Lanes G, A, T, and C show the respective sequencing products resulting from a sequencing reaction with oligonucleotide 5-1 and with ddGTP, ddATP, ddTTP, and ddCTP, respectively. The first lane contains the primer extension product, which is indicated by an arrow. In the sequence represented on the right, the corresponding transcription start site is indicated with an asterisk.
