TABLE 1.
Purification step | Vol (ml) | Protein (mg/ml) | Ribulose 5-P reductase sp act (μmol/min/mg) | CDP-ribitol PPase sp act (μmol/min/mg) | Recovery (%)
|
|
---|---|---|---|---|---|---|
Reductase | PPase | |||||
Crude extract | 48 | 6.25 | 1.88 | 0.22 | 100.0 | 100.0 |
DEAE-Sepharose | 14 | 4.57 | 5.60 | 1.42 | 63.5 | 137.7 |
Blue-Sepharose | 4 | 2.48 | 37.50 | 15.70 | 65.8 | 235.5 |
Elution of DEAE- and Blue-Sepharose columns was performed as described in Materials and Methods and the legend to Fig. 5. PPase, pyrophosphorylase; 5-P, 5-phosphate.