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. 1999 Apr;181(7):2001–2007. doi: 10.1128/jb.181.7.2001-2007.1999

TABLE 1.

Purification tablea

Purification step Vol (ml) Protein (mg/ml) Ribulose 5-P reductase sp act (μmol/min/mg) CDP-ribitol PPase sp act (μmol/min/mg) Recovery (%)
Reductase PPase
Crude extract 48 6.25 1.88 0.22 100.0 100.0
DEAE-Sepharose 14 4.57 5.60 1.42 63.5 137.7
Blue-Sepharose 4 2.48 37.50 15.70 65.8 235.5
a

Elution of DEAE- and Blue-Sepharose columns was performed as described in Materials and Methods and the legend to Fig. 5. PPase, pyrophosphorylase; 5-P, 5-phosphate.