FIG. 4.

(i) ccm locus. ccmA, curved cell morphology; pat, putative acetyltransferase; ygbA, homolog of E. coli ygbA; virF, homolog of Y. pestis virF; Tn, site of transposon insertion; C, ClaI; H, HindIII; EV, EcoRV; S, SacII; N, NsiI; X, XbaI; Sp, SpeI; P, PstI; B, BglII. The box indicates the region of sequence shown in panel ii. (ii) DNA and protein sequences for the ccm locus. Codons are numbered from the first ccmA Met residue, where CcmA1 presumably initiates. The first three Met residues are indicated in bold. Met 59 is the probable N terminus of CcmA2. Tn, transposon insertion. Primers used to amplify ccmA and incorporate EcoRI, BglII, and HindIII sites are indicated by broken arrows. (iii) Hydrophobicity plot (33), showing positions at which a shorter CcmA initiates translation (M59) and Tn196 is inserted. (iv) Hybridization of a ccmA probe to total RNA from wild-type P. mirabilis cells harvested 4 h after seeding. RNA markers are indicated in kilobases.