TABLE 2.

Expression of P_his and his leader region-lux fusions on LC plasmids or integrated on the chromosome

Plasmidsa	Promoter and leader regionb upstream of lux AB genes	Luciferase activities (lx/OD [103])		Strength of histidine regulation (fold)d
		CDM+H	CDM−H
pProm-1	WTc	12 ± 3	120 ± 40	10
pInt-1		2.8 ± 0.9	335 ± 50	120
pInt-1m2	Deletion of 46 bp upstream of Phis (nt 632 to 677)	2.7 ± 0.3	290 ± 40	110
pInt-6	Deletion from nt 831 downstream of Phis	230 ± 85	200 ± 30	0.9
pProm-7	Deletion from nt 948 downstream of Phis	355 ± 25	390 ± 40	1.1
pInt-7		265 ± 20	235 ± 60	0.9
Pprom-1m3	Substitution of 14 bp; overlaps the terminator and TATTAT box	150 ± 25	125 ± 35	0.8
pInt-1m3		140 ± 40	270 ± 40	1.9
pInt-1m4	Substitution of 8 bp; overlaps the terminator	190 ± 60	330 ± 40	1.7

^aThe lux fusions are on an LC plasmid for the pProm series or integrated on the chromosome for the pInt series. 

^bSee Fig. 1. 

^cWT, the P_his and his leader region from wild-type fragment. 

^dObtained by the division of the values obtained in the presence of histidine by those obtained in the absence of histidine for the corresponding plasmid.