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. 2022 Aug 5;3(3):101602. doi: 10.1016/j.xpro.2022.101602

Figure 2.

Figure 2

Kinetic assessment of ΔΨm

(A) Continuous monitoring of the ΔΨm over time and assessment of the robustness of the assay (Z′ score) in NPCs treated with DMSO or with FCCP and antimycin A (AA). iPSC-derived NPCs were stained with 10 nM TMRM (in non-quenching mode) for 30 min followed by a washing step and HCS-based quantification of ΔΨm.

(B) Quantification of ΔΨm intensity based on the TMRM signal in iPSC-derived NPCs treated with DMSO or with FCCP and antimycin A (AA) at dedicated time points. Mean ± SD, unpaired Student’s t-test, ∗∗p < 0.01.