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NPC growth medium (sm+)
| Medium | Component | Amount | Stock con. | Final conc. |
|---|---|---|---|---|
| Growth medium for NPCs (sm+) | sm- | 10 mL | 1 × | 1 × |
| CHIR99021 (Glycogen synthase kinase (GSK)-3 inhibitor) | 5 μL | 6 mM | 3 μM | |
| Purmorphamine | 7.69 μL | 0.65 mM | 500 nM | |
| Vitamin C | 7.5 μL | 200 mM | 150 μM |
