NPC basal medium (sm−) for live-cell imaging

Medium	Component	Amount	Stock concentration	Final concentration
Phenol red-free basal medium for live-cell imaging of NPCs	DMEM/F12 without phenol red	240 mL	1 ×	0.5 ×
	Neurobasal without phenol red	240 mL	1 ×	0.5 ×
	N2	2.5 mL	100 ×	0.5 ×
	B27 w/o Vitamin A	5 mL	50 ×	0.5 ×
	Glutamine	5 mL	200 mM	2 mM
	MycoZap	1 mL	500 ×	1 ×
	[Pen/Strep∗]	[5 mL]	[100 ×]	[1 ×]