TABLE 1.
Effects of pil mutations on transfer frequency and sensitivity to phages
| Plasmid | Mutationa
|
Transfer frequency (%)b | Sensitivity to phagec:
|
||
|---|---|---|---|---|---|
| Location | Length (bp) | Iα | PR64FS | ||
| pKK641A′ | 1.2 | ||||
| pKK641A′ (mut-6) | 3713 | +22 | 1.0 | + | + |
| pKK641A′ (pilI1) | 3893 | +22 | 1.3 | + | + |
| pKK641A′ (pilJ1) | 4075 | +22 | 1.1 | + | + |
| pKK641A′ (pilJ2) | 4189 | +22 | 1.3 | + | + |
| pKK641A′ (pilK1) | 4566 | +22 | 0.005 | − | − |
| pKK641A′ (pilK2) | 5000 | −4 | 0.002 | − | − |
| pKK641A′ (pilL1) | 5510 | +22 | 0.002 | − | − |
| pKK641A′ (pilL2) | 5682 | +22 | 0.002 | − | − |
| pKK641A′ (pilM1) | 6266 | +22 | 0.003 | − | − |
| pKK641A′ (pilM2) | 6413 | +22 | 0.003 | − | − |
| pKK641A′ (pilN1) | 6854 | +22 | 0.004 | − | − |
| pKK641A′ (pilN2) | 7238 | +22 | 0.002 | − | − |
| pKK641A′ (pilO1) | 8719 | +22 | 0.002 | − | − |
| pKK641A′ (pilO2) | 9189 | −4 | 0.003 | − | − |
| pKK641A′ (pilP1) | 9798 | +22 | 0.002 | − | − |
| pKK641A′ (pilQ1) | 10201 | +22 | 0.002 | − | − |
| pKK641A′ (pilQ2) | 10467 | +22 | 0.001 | − | − |
| pKK641A′ (pilR1) | 11676 | −4 | 0.9 | + | + |
| pKK641A′ (pilR2) | 11873 | +22 | 0.002 | − | − |
| pKK641A′ (pilS1) | 12807 | +22 | 0.002 | − | − |
| pKK641A′ (pilS2) | 12945 | +22 | 0.003 | − | − |
| pKK641A′ (pilT1) | 13433 | +22 | 0.013 | + | + |
| pKK641A′ (pilT2) | 13551 | +22 | 0.018 | + | + |
| pKK641A′ (pilU1) | 14381 | +22 | 0.002 | − | − |
| pKK641A′ (pilV1) | 15254 | +22 | 0.003 | − | − |
a
Locations (nucleotide number in the DNA sequence of GenBank accession no. D88588) and lengths (+, insertion; −, deletion) of the mutations are indicated.
b
Transfer frequency of pKK661 from donor cells harboring the indicated plasmids and pKK661 in liquid mating is indicated as a percentage of transconjugants relative to donor cells.
c
Sensitivity to phages Iα and PR64FS of E. coli cells harboring the indicated plasmid (+, sensitive; −, resistant).