TABLE 2.
Complementation of pil mutations by pil+ plasmids
| Plasmid | Complementation with plasmid froma:
|
|
|---|---|---|
| pKK696a series | pKK696b series | |
| pKK641A′ | ||
| pKK641A′ (pilK1) | + (pKK696a) | + (pKK696b) |
| pKK641A′ (pilL1) | + (pKK697a) | + (pKK697b) |
| pKK641A′ (pilM1) | + (pKK698a) | + (pKK698b) |
| pKK641A′ (pilN1) | + (pKK699a) | + (pKK699b) |
| pKK641A′ (pilO1) | + (pKK700a) | + (pKK700b) |
| pKK641A′ (pilP1) | + (pKK701a) | + (pKK701b) |
| pKK641A′ (pilQ1) | + (pKK702a) | + (pKK702b) |
| pKK641A′ (pilR2) | + (pKK703a) | + (pKK703b) |
| pKK641A′ (pilS1) | + (pKK692a) | + (pKK692b) |
| pKK641A′ (pilT1) | − (pKK704a) | − (pKK704b) |
| pKK641A′ (pilU1) | + (pKK705a) | + (pKK705b) |
| pKK641A′ (pilV1) | Unstable (pKK706a) | + (pKK706b) |
a
Transfer frequency of pKK661 from donor cells harboring pKK661 and the indicated plasmids with complementing plasmids (indicated in parentheses) in liquid matings was estimated as in Table 1. +, with complementation activity (transfer frequencies recovered to a level similar to that of wild-type pKK641A′); −, without complementation activity.