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. 2022 Aug 9;20:176. doi: 10.1186/s12915-022-01381-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of CDZ binding on the deuterium uptake profile of holo-CaM. A, B Relative fractional uptake plots of holo-CaM measured in the presence and in the absence of 20 μM CDZ. Each dot corresponds to the average uptake value measured in three independent replicates. C The effects of CDZ binding on holo-CaM are visualized on the fractional uptake difference plot. Negative values indicate a reduction in solvent accessibility induced by CDZ binding. D Cartoon representation of holo-CaM showing the average differences in “fractional uptake differences” between the CDZ-bound and free holo-CaM states. The fractional uptake differences ([ΔDeuteration] in %) measured between the CDZ-bound and free states were extracted for each peptide at each labelling time point, averaged, and plotted on the crystal structure of CaM:CDZ_A (PDB ID: 7PSZ). CDZ-A is colored in green. The average ΔDeuteration values [Average (ΔDeuteration)] are colored from blue (no variation) to red (major reductions in uptake)