Fig. 6.

Genome-wide binding events of the menin mutants. A Correlation of the coverage between WT menin and menin mutants. Values represent Pearson correlations of normalized coverage calculated for 10-kb bins. B Coverage tracks of the regions bound by menin WT and menin mutants. The left box highlights a genomic region occupied by both menin WT and mutant proteins, while the right box highlights a genomic region where binding of the menin mutants R52G, E255K and E359K was lost. C Percentages of peaks retained at menin WT bound sites by greenCUT&RUN mapping using the indicated menin mutant. In blue, retained binding at menin–JunD sites without MLL1/H3K4me3 is shown. In red, retained binding of mutant forms of menin at WT menin–MLL1/H3K4me3 sites without JunD is shown. D Heatmaps showing coverage on the retained (upper panel) and lost (lower panel) peaks with menin mutants relative to WT menin. The number of the peaks are given on the left side. The coverage is shown on the top of each heatmaps. Please note that the E408Q mutant essentially behaves like WT menin