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. 2022 Aug 5;221(9):e202112097. doi: 10.1083/jcb.202112097

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Figure S1. — Centriole motility is independent of the Actin network and centrioles associate with the interphase MT cytoskeleton. (A) Z-stack projection of example PC’s labelled with Phalloidin showing that 10 µM Latrunculin-A treatment destroys the Actin network. Scale bars: 10 μm. (B) Tracks showing the movement of centrioles over a 10-min period. Latrunculin-A treatment does not inhibit centriole motility in peripodial cells. Scale bars: 5 μm. (C) Quantification of average velocity. (DMSO: 117.6 nm/s ± 34, n = 126, Lat-A: 123.5 nm/s ± 41, n = 140. Data = mean ± SD. Unpaired, two tailed, t test: P = 0.19). (D) Mean squared displacement is not affected by Latrunculin A treatment. Data = Mean ± SD (D) Timelapse series of an S2 cell labelled with SiR-Tubulin. Arrowhead denotes brighter spot corresponding to the centriole moving along the MT network. Scale bar: 2 μm. (E) Projection of a live S2 cell transfected with PACT::GFP to label the centriole (magenta). Centriolar signal is coincident with bright accumulation of SiR-Tubulin (green). Scale bar: 10 μm, inset: 1 μm. (F) Live PC expressing SAS-4::GFP (centriole, magenta) and labelled with SiR tubulin (green). SiR tubulin accumulation corresponds to SAS-4 positive centrioles. Scale bar: 5 μm; inset: 1 μm.