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. 2022 Aug 5;221(9):e202112097. doi: 10.1083/jcb.202112097

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Figure S2. — PLP knockdown does not cause precocious centriole activation in PCs. (A) Fixed PCs stained for Asl (red), Cnn (cyan), and Gamma-tubulin (green) shows that PCM does not accumulate in PLPRNAi expressing PCs. (B) Quantification of Centrosomin intensity relative to cytoplasm (Control: 1.16 ± 0.05 n = 7 wing discs, 67 centrioles; PLPRNAi²⁹⁶: 1.1 ± 0.05, n = 8 wing discs, 91 centrioles; PLPRNAi⁶⁴⁵: 1.1 ± 0.05, n = 8 wing discs, 86 centrioles. Mean ± SD. ANOVA: P = 0.003. Dunnett’s multiple comparison: Control vs PLPRNAi²⁹⁶: P = 0.0035, Control vs PLPRNAi⁶⁴⁵: P = 0.0065). (C) Quantification of Gamma Tubulin intensity relative to cytoplasm (Control: 1.15 ± 0.05 n = 7 wing discs, 67 centrioles; PLPRNAi²⁹⁶: 1.1 ± 0.04, n = 8 wing discs, 91 centrioles; PLPRNAi⁶⁴⁵: 1.2 ± 0.06, n = 8 wing discs, 86 centrioles. ANOVA: P = 0.01, multi-comparison test showed no significance between Control and RNAi groups. Mean ± SD). (D) Fixed mitotic wing disc cells stained for Asl (red), Cnn (cyan), and Gamma-tubulin (green). PLP knockdown does not prevent PCM accumulation in mitosis. But PCM can appear disorganized. Scale bars: 1 µm.