Video 18.
Age-dependent centrosome segregation is disrupted following PLP or KHC knockdown. Spinning disk confocal time lapse showing maximum projection of isolated neuroblasts expressing ubi-Cbn::GFP (daughter centriole, cyan, Arrowhead) and UAS-mCherry::Jupiter (MTs, red). Note that following PLP (center) or KHC (right) knockdown the Cbn + centriole ends up segregating to the basal side of the cell in mitosis rather than being retained by the NB at the apical side. Time stamp: hh:mm. Scale bar: 5 µm.