FIG. 5.
Complete nucleotide sequence of the 1.75-kb PstI fragment containing the haloalkane dehalogenase gene. The deduced amino acid sequence of the dehalogenase open reading frame is indicated below the corresponding DNA sequence. The three amino acid substitutions and the 14-amino-acid extension at the C terminus, which are the differences compared to the DhaA amino acid sequence, are shown in boldface. The sequence upstream of dhaAf identical to the sequence upstream of the dhaA gene in R. rhodochrous NCIMB 13064 is underlined. The position of the deletion of 12 nucleotides, compared to the dhaA gene region in strain NCIMB 13064, between nucleotides 49 (T) and 50 (G) is indicated by an arrow. The sequence identical to the hheB gene region in Corynebacterium sp. strain N-1074 is indicated by a double underline.