TABLE 2.
Dehalogenase activities in crude extracts from strain GP1
| Substrate | Dehalogenase sp acta (mU/mg of protein)
|
Product | |
|---|---|---|---|
| 1,2-DBE | 1-Propanol | ||
| 1,2-Dibromoethane | 220 | 230 | 2-Bromoethanol |
| 1,2-Dichloroethane | <30 | <30 | |
| Bromochloroethane | 180 | NDb | 2-Chloroethanol |
| 1-Chloropropane | 50 | ND | 1-Propanol |
| 1-Bromobutane | 30 | ND | 1-Butanol |
| 1,3-Dibromopropane | 80 | 80 | ND |
| 2-Bromoethanol | 5,200 | 8,110 | Ethylene oxide |
| 2-Chloroethanol | 70 | 110 | Ethylene oxide |
| 3-Chloro-1,2-propanediol | ND | 300 | ND |
| 2,3-Dichloro-1-propanol | ND | <30 | |
| 1,3-Dichloro-2-propanol | ND | 5,220 | Epichlorohydrin |
| 1-Chloro-2-propanol | ND | 3,260 | Propylene oxide |
| Bromoacetate | <30 | <30 | |
| Chloroacetate | <30 | <30 | |
| Dibromoacetate | <30 | <30 | |
| Dichloroacetate | <30 | <30 | |
Specific activities with various substrates (5 mM) were determined with extracts prepared from cells grown with either 1,2-dibromoethane or 1-propanol.
ND, not determined.