Fig. 1.

Microscopy images indicating that cells are intact with the DNA within the cells after fixation. A Confocal microscopy images of acetone fixed bacteria stained with acridine orange (AO), setting adjusted to allow observation of DNA, RNA, differential interference contrast (DIC), and an overlay of all three. Images presented are maximum intensity projections of confocal Z stacks. B EM images of bacterial cells, black arrows indicate the presence of granular nucleoplasm with fibrillar whorls inside the cell membranes, indicating that DNA is preserved within the cells post fixation. White arrows indicate the presence of cell walls