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. 2022 Aug 9;10:123. doi: 10.1186/s40168-022-01313-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Comparison of selected DNA extraction kits when using mock communities of different microbial compositions. Kits selected were Kit 2, Kit 6, and Kit 7. Data is based on shotgun sequencing of DNA analysed using MetaPhlAn3. Mock communities used were NIBSC WC-Gut RR, ZymoBIOMICS Microbial Community Standards (ZYMO), 20 Strain Even Mix Whole Cell Material (ATCC-MSA 2002), and 10 Strain Even Mix Whole Cell Material (ATCC-MSA 2003). A Sensitivity. B Similarity in composition with the actual composition. C False-positive relative abundance (FPRA). D Relative abundance of gram-positive and gram-negative bacteria in the samples. The results are based on the average of three replicates