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Strains used in the WC-Gut RR, their DSMZ culture collection number, the gram stain (+ for positive and − for negative), the genome size (bp), the 16S rRNA copy number, the amount of ng added in the reagent (serving as ground truth for the WC-Gut RR), the genome size adjusted composition (%) (serving as ground truth for the DNA-Gut-Mix RR), and the relative composition when adjusted for 16S copy number (%). 16S rRNA copy number is based on analysis of genome sequences through IMG/M
