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. 1999 Apr;181(7):2059–2066. doi: 10.1128/jb.181.7.2059-2066.1999

FIG. 5.

FIG. 5

Nucleotide sequence of the ptb upstream region. Deletion end points are indicated by arrows and numbered with respect to the transcriptional start site (indicated by asterisks). The sequences at positions −12 and −24 are indicated. Boxed regions indicate putative UAS. The effects of upstream deletions on the expression of the ptb′-′lacZ translational fusions are expressed as β-galactosidase specific activities, which were determined with extracts prepared from cells growing exponentially in MM minimal medium containing glucose and 20 mM isoleucine as the inducer. In the absence of isoleucine, the basal level was about 5 U/mg of protein for each strain. For deletion end points ΔA (−232), ΔB (−122), ΔC (−107), ΔD (−92), and ΔE (−77), β-galactosidase activities were 2,350, 2,135, 205, 5, and 10 U/mg of protein, respectively.

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