TABLE 1.
B. subtilis strains used in this study
| Strain | Genotype or descriptiona | Source or referenceb |
|---|---|---|
| 168 | trpC2 | 1 |
| QB5505 | trpC2 sigL::aphA3 | 5 |
| QB7501 | trpC2 amyE::ptb′-′lacZ (ΔA) | This work |
| QB7502 | trpC2 amyE::ptb′-′lacZ sigL::aphA3 | QB5505 DNA→QB7501 |
| QB7505 | trpC2 amyE::ptb′-′lacZ tnrA::erm | SF706T DNA→QB7501 |
| QB7507 | trpC2 lpd::pMutin4 | This work |
| QB7511 | trpC2 amyE::ptb′-′lacZ bkdR::aphA3 | pBkdR2 lin→QB7501 |
| QB7512 | trpC2 bkdR::aphA3 | pBkdR2 lin→168 |
| QB7513 | trpC2 lpd::pMutin4 bkdR::aphA3 | pBkdR2 lin→QB7507 |
| QB7514 | trpC2 bcd::pMutin4 | This work |
| QB7515 | trpC2 bcd::pMutin4 bkdR::aphA3 | pBkdR2 lin→QB7514 |
| QB7517 | trpC2 amyE::ptb′-′lacZ (ΔB) | This work |
| QB7518 | trpC2 amyE::ptb′-′lacZ (ΔC) | This work |
| QB7519 | trpC2 amyE::ptb′-′lacZ (ΔD) | This work |
| QB7520 | trpC2 amyE::ptb′-′lacZ (ΔE) | This work |
| QB7521 | trpC2 bkdB::pMutin4 | This work |
| QB7522 | trpC2 amyE::ptb′-′lacZ codY::erm | PS258 DNA→QB7501 |
| QB7530 | trpC2 amyE::ptb′-′lacZ bkdR::aphA3 pBkdR1 | This work |
| PS258 | trpC2 codY::erm | A. L. Sonenshein |
| SF706T | trpC2 tnrA::Tn917 amyE::gabP-lacZ | 38 |
a
ΔA, ΔB, ΔC, ΔD, and ΔE are defined in Fig. 5.
b
lin, linearized. pBkdR1 and pBkdR2 were constructed as discussed in Materials and Methods.