TABLE 4.
Induction of the expression of bcd, lpd, and bkdB genes
Strain | Relevant genotype | β-Galactosidase activity (Miller units/mg of protein) in the following minimal mediuma:
|
|||
---|---|---|---|---|---|
MM + glucose
|
MM + glucose + isoleucine (20 mM)
|
||||
Without IPTG | With IPTG | Without IPTG | With IPTG | ||
QB7514 | bcd::pMutin4 | 355 | 10 | 1,170 | 1,100 |
QB7515 | bcd::pMutin4 bkdR::aphA3 | 2 | 2 | 3 | 2 |
QB7507 | lpd::pMutin4 | 320 | 280 | 2,800 | 2,630 |
QB7513 | lpd::pMutin4 bkdR::aphA3 | 5 | 5 | 3 | 4 |
QB7521 | bkdB::pMutin4 | 290 | ND | 1,150 | ND |
ND, not determined.