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. Author manuscript; available in PMC: 2022 Aug 9.
Published in final edited form as: Methods Mol Biol. 2022;2507:375–387. doi: 10.1007/978-1-0716-2368-8_20

Fig. 3: Purification of SapCF in polymer SMALP 200P.

Fig. 3:

After affinity purification (A) SapF and (B) SapC were run on a size exclusion column, each resulting in a single peak at 95 % purity as indicated by SDS-PAGE. Both proteins were mixed to create a (C) SapCF complex before running on the size exclusion column to ensure complex formation of the transporter.