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. Author manuscript; available in PMC: 2022 Aug 9.
Published in final edited form as: FASEB J. 2022 Apr;36(4):e22242. doi: 10.1096/fj.202100901RRR

FIGURE 2.

FIGURE 2

Multivariate analysis and hierarchical clustering displays statistical metabolite differences between WT and Rbp1−/− mice atria, LV and RV. (A) PLS-DA plot comparing Rbp1−/− Atria (Red), Rbp1−/− LV (Green), Rbp1−/− RV (Blue), WT Atria (Cyan), WT LV (Magenta), WT RV (Yellow). R2 = 0.98. Q2 = 0.91. N = 5 per group. Each point represents a data set from an individual animal tissue. The 95% confidence intervals are indicated by elliptical patterns per group. (B) PLS-DA plot comparing all Rbp1−/− samples (black) and WT samples (gray). R2 = 0.99. Q2 = 0.96. n = 15 per group. The 95% confidence intervals are indicated by elliptical patterns per group. (C) Heatmap displaying the top 25 metabolites based on t-test/ANOVA, Euclidean distancing, and Ward clustering