TABLE 2.
Genotypic and phenotypic characterization of N. meningitidis clinical isolates and isogenic hpuB::erm and hmbR::kan derivatives
| Serotype | Clinical isolates
|
hpuB::erm derivatives
|
hmbR::kan derivatives
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain | hmbRa | hpuBa | PHb | Strain | No. of Gc | PH | Strain | PH | |
| C | 2857 | + | + | LW | 3272 | 8 | SC | 3303 | LW |
| 2858 | + | + | LW | 3273 | 13 | SC | 3304 | LW | |
| 2859 | + | + | LW | 3274 | 12 | LW | 3305 | SC | |
| 2860 | + | + | LW | 3275 | 10 | SC | 3306 | LW | |
| 2861 | + | + | LW | NTd | * | NA | NT | NA | |
| 2862 | + | + | LW | 3276 | 13 | SC | 3307 | LW | |
| 2863 | + | + | LW | 3277 | 13 | SC | 3308 | LW | |
| A | 2848 | + | + | LW | 3265 | 9 | LW | NT | NA |
| 2849 | + | + | LW | NT | 9 | NA | 3299 | SC | |
| 2850 | − | + | LW | 3266 | * | 0 | NA | LW | |
| 2851 | + | + | LW | 3267 | 9 | LW | 3300 | SC | |
| 2852 | − | + | LW | 3268 | * | 0 | NA | LW | |
| 2853 | − | + | LW | 3269 | * | 0 | NA | LW | |
| 2854 | − | + | LW | NT | * | NA | NA | LW | |
| 2855 | + | + | LW | 3270 | 8 | SC | 3301 | SC | |
| 2856 | + | + | LW | 3271 | 9 | LW | 3302 | SC | |
| Y | 2843 | + | + | LW | NT | 10 | NA | NT | NA |
| 2844 | − | + | 0 | 3262 | * | 0 | NA | 0 | |
| 2845 | − | + | LW | 3263 | * | 0 | NA | LW | |
| 2846 | − | + | LW | 3264 | * | 0 | NA | LW | |
| 2847 | + | + | LW | NT | 10 | NA | NT | NA | |
| B | 1074 | + | − | LW | NAe | 9 | LW | 3297 | 0 |
| 2781 | + | + | SC | 3261 | 8 | SC | 3298 | SC | |
a
Presence (+) or absence (−) of homologous sequence determined by Southern blot hybridization.
b
The Hb utilization phenotype (PH) of the strain is as follows: LW, confluent lawn; SC, single colony; NA, not applicable; 0, no growth.
c
Number of consecutive G residues in the poly(G) tract of hmbR. An asterisk indicates that no sequence was obtained.
d
NT, no transformants obtained.
e
NA, not available.