TABLE 3.

List of primers used for identification confirmation, classification and resistance gene detection in E. coli isolates

Gene	Size (bp)	Primer	Sequences (5′–3′)	References/notes
uidA	192	Forward	CAGTCTGGATCGCGAAAA	27
		Reverse	ACCAGACGTTGCCCACATA
16S rRNA	357	Forward	AGAGTTTGATCCTGGCTCAG	47 48
		Reverse	TGACGGGCGGTGTGTACAAG
chuA	279	Forward	GACGAACCAACGGTCAGGAT	10
		Reverse	TGCCGCCAGTACCAAAGACA
yjaA	211	Forward	TGAAGTGTCAGGAGACGCTG
		Reverse	ATGGAGAATGCGTTCCTCAAC
TspE4.C2	152	Forward	GAGTAATGTCGGGGCATTCA
		Reverse	CGCGCCAACAAAGTATTACG
bla CTX-M	593	Forward	ATGTGCAGYACCAGTAARGT	49; designed from conserved regions of blaCTX-M genes, including blaCTX-M-1 to blaCTX-M-30
		Reverse	TGGGTRAARTARGTSACCAGA
bla TEM	964	Forward	GCGGAACCCCTATTTG	50
		Reverse	ACCAATGCTTAATCAGTGAG
bla SHV	854	Forward	TTCGCCTGTGTATTATCTCCCTG	51
		Reverse	TTAGCGTTGCCAGTGYTCG
bla CMY-1	915	Forward	GTGGTGGATGCCAGCATCC
		Reverse	GGTCGAGCCGGTCTTGTTGAA
bla CMY-2	758	Forward	GCACTTAGCCACCTATACGGCAG
		Reverse	GCTTTTCAAGAATGCGCCAGG
bla OXA-1	820	Forward	ATGAAAAACACAATACATATCAACTTCGC	50
		Reverse	GTGTGTTTAGAATGGTGATCGCAT
bla OXA-2	602	Forward	ACGATAGTTGTGGCAGACGAAC	51
		Reverse	ATYCTGTTTGGCGTATCRATATTC