FIG 1.

Potential binding sites of CumDO-F on CumDO-O. Two potential binding sites were predicted by docking simulations using GRAMM-X. In panels A and B, CumDO-F models based on the crystal structures of the TDO-F (PDB entry 4EMJ) (37) were used, while in panel C, the CumDO-F model prepared from BDO-F (PDB entry 1FQT) (8) was used. The surfaces of the α- and β-subunits of CumDO-O (PDB entry 1WQL) are shown in magenta and green, respectively. Homology-modeled CumDO-Fs are shown in blue in the ribbon model. (A) Binding at the α-subunit interface at the top-wise site and (B and C) binding at the α- and β-subunit boundary at the side-wise site are shown.