FIG 3.

Reduction efficiencies of alanine-substituted CumDO-Os by CumDO-F. Panels A and B show the reduction efficiencies of single-alanine-substituted CumDO-Os and double-alanine-substituted CumDO-Os, respectively. Results for WT CumDO-O (set at 100%) are shown as gray bars. Black and white bars are the reduction efficiencies of CumDO-O derivatives with amino acid substitutions at the top-wise and side-wise potential CumDO-F-binding sites, respectively. Error bars indicate standard deviations from three independent experiments. The data were assessed using Student's t test with P values of <0.005 (**) or <0.001 (***) for alanine-substituted CumDO-Os compared to the WT.