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Average kcat/Km values for hydrolysis of antibiotics. Values were estimated for hydrolysis of ampicillin (A), ceftiofur (B), DFC (C), DFC-cysteine (D), and DFC-dimer (E) using recombinant CMY-2, CTX-M-15, and KPC-3 enzymes. See Materials and Methods for additional details and Table 1 and Fig. S7 and S8 for supporting information.
