Figure 2.

Binding of CipA to different complement components of the AP and CP. Protein binding of C3 (A), C3b (B), C3c (C), C5 (D), C4b (E), FB (F), FD (G), and FH (H), respectively, to CipA to was measured by ELISA. CipA (5 ng/µl) was immobilized and incubated with 5 ng/µl purified complement components and BSA or gelatine were used as negative controls (ctrl). Bound complement components were detected using specific antisera (1:1,000). To assess statistical significance, two-tailed, unpaired Student’s t-test was performed. Data represent means and/or standard deviation of at least three different experiments, each conducted in at least triplicate. **, p ≤ 0.05; ****, p ≤ 0.0001; n.s., no statistical significance.