TABLE 1.
Colony size in the presence of puromycina
| Strain | Colony diam, in mm, after incubation with the following puromycin concn:
|
|||||
|---|---|---|---|---|---|---|
| 0 | 10 | 14 | 20 | 14 (2 days) | 20 (2 days) | |
| MG1363 (wild type) | 1.2 ± 0.1 | 0.9 ± 0.1 | 0.8 ± 0.1 | 0.12 ± 0.02 | 1.1 ± 0.2 (2.5 × 108) | 0.6 ± 0.3 (2.0 × 107) |
| HI1635 (clpBΔ1) | 1.3 ± 0.1 | 0.9 ± 0.1 | 0.7 ± 0.05 | 0.30 ± 0.09 | 1.1 ± 0.1 (2.1 × 108) | 1.0 ± 0.1 (1.4 × 108) |
| HI1632 (clpCΔ1) | 1.2 ± 0.1 | 0.9 ± 0.1 | 0.6 ± 0.05 | 0.14 ± 0.03 | 1.2 ± 0.1 (2.4 × 108) | 1.1 ± 0.1 (1.2 × 108) |
| HI1615 (clpEΔ1) | 1.2 ± 0.1 | 0.9 ± 0.1 | 0.15 ± 0.02 | 0.04 ± 0.01 | 1.1 ± 0.1 (1.2 × 108) | 0.07 ± 0.04 (ND) |
a
Wild-type and mutant cells were plated in the presence of the indicated puromycin concentrations. After growth at 30°C (for 1 day, unless otherwise indicated), the colony diameter was determined. The numbers are the average ± standard deviation for at least 15 determinations. After 2 days of incubation, the numbers of colonies were also determined (numbers in parentheses; ND indicates that the colonies were too small to count).