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| Item | Penalty points |
|---|---|
| 1. Analytical eco-scale score 26 | |
| (1) Reagent; volume (ml) | |
| Ethanol; <10 ml | 4 |
| Acriflavine; 1 ml | 6 |
| BR buffer; 1.2 ml | 2 |
| (2) Spectrofluorimeter; <0.1 KW h per sample | 0 |
| (3) Occupational hazard | 0 |
| (4) Waste | 3 |
| Total penalty points | 15 |
| Analytical eco-scale score | 85 |
| 2. Green analytical procedure index (GAPI) 27 | |
|
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