TABLE 1.

Purification of the GST and dehydrogenase from Rhodococcus sp. strain AD45

Step	Total protein (mg)	Total activity (U)	Sp act (U mg of protein−1)	Purification factor	Yield (%)
GST
Cell extract	188	959	5.1	1	100
(NH4)2SO4	233	932	4.0	0.8	97
Resource Q	60	786	13.1	2.6	82
Resource Iso	8.9	594	66.7	13.1	62
Dehydrogenase
Cell extract	336	1,001	2.9	1	100
DE52	96	932	6.3	2.2	93
Sephacryl S-300	42	786	8.0	2.8	79
Blue Sepharose	15	198	13.3	4.6	20
Resource Iso			18.0	6.2